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The instantaneous frequencies of action potentials occurring on the waveform selected by the "Spike W.F. #" are displayed with respect to the corresponding levels on the waveform selected by the "Amplitude W.F. #". That is, for each spike on the former waveform, the instantaneous frequency (inverse of the interval from the previous spike) is calculated, and plotted on the graph at the position representing the level on the latter waveform. The parameters "W.F. amplitude delay" and "W.F. amplitude window" indicate a range of points, relative to the point where the action potential occurred, used to obtain a level reading on this latter waveform. The range of voltage levels can be restricted to a smaller range, by setting the parameters "Min W.F. amplitude" and "Max W.F. amplitude". Only action potentials whose associated waveform level readings stay within these bounds will be included in the graph.
No frequency is plotted for the first spike in the selected range to be analysed, since a previous spike is needed to permit an interval calculation. If the spike trains for the former waveform have been properly set, frequencies are calculated and plotted only for spikes in the same spike train, and no frequency is plotted for the first spike in each train.
If the "Take interval after spike" option is enabled, the interval to the following spike, rather than the previous one, is calculated. No frequency is plotted for the last spike in the range to be analysed, nor for the last spike in each train, when spike trains are set.